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Education Courses Atomspectroscopic methods in the determination of soil, water, fodder, biological samples

Atomspectroscopic methods in the determination of soil, water, fodder, biological samples

Language
English
Nature
optional C
Method of evaluation
semi-final examination
Year in the curriculum
4
Semester in the curriculum
8
Credits
2
Lectures
9
Practical lessons
6
Allow for
  • Vet EN

Course description

General target of the subject to familiarize the students of the most important methods of element analysis, the sample preparation methods and the most important points of the evaluation of the results (detection limit, quantification limit, accuracy). For the vets it is very important to be familiar with the performance parameters of the different analytical techniques, to be able to estimate the reliability of the measured data.

Lectures theme

1.    Average concentration of important major, minor and trace elements in soils, rocks, natural waters, animal and human bodies.

2.    Introduction of atomspectrometric methods I. (SF, AAS, AES). Detailed discussion of the methods, different applications.

3.    Introduction of atomspectrometric methods II. (ICP-OES). Detailed discussion of the methods, different applications.

4.    Introduction of atomspectrometric methods III .( ICP-MS, XRF). Detailed discussion of the methods, different applications.

5.  Presentation of instrument analytical techniques in the ICP-OES and AAS laboratory of the Department of Animal Hygiene, Herd Health and Veterinary Ethology. Determination of biological samples (blood, blood plasma, serum, tissue samples from animals).

6.   Practice: Sample preparation of soil, water and biological samples for the determination of their macro and micro elements.

7.    Practice: Determination of Se and Mn content of blood samples by Perkin Elmer PinAAcle 900Z THGA AAS instrument.

8.  Practice: Principle of operation of Perkin- Elmer Optima 8300 DV ICP-OES instrument. Possibilities of method development.