{"id":11103,"date":"2016-02-04T16:00:00","date_gmt":"2016-02-04T15:00:00","guid":{"rendered":"http:\/\/univet.hu\/research\/topics\/prevention-of-oxidative-stress-induced-changes-of-xenobiotic-metabolism-investigated-in-a-co-culture-model-of-pig-intestinal-and-hepatic-cells\/"},"modified":"2016-02-04T16:00:00","modified_gmt":"2016-02-04T15:00:00","slug":"prevention-of-oxidative-stress-induced-changes-of-xenobiotic-metabolism-investigated-in-a-co-culture-model-of-pig-intestinal-and-hepatic-cells","status":"publish","type":"page","link":"https:\/\/univet.hu\/en\/research\/topics\/prevention-of-oxidative-stress-induced-changes-of-xenobiotic-metabolism-investigated-in-a-co-culture-model-of-pig-intestinal-and-hepatic-cells\/","title":{"rendered":"Prevention of oxidative stress-induced changes of xenobiotic metabolism investigated in a co-culture model of pig intestinal and hepatic cells"},"content":{"rendered":"<p>Investigations with cell co-culture model systems are needed to understand the complex interrelationships between such processes as intestinal absorption, bioavailability and hepatic biotransformation in the animal organism. However, at present, suitable animal co-culture models mimicking the in vivo enterohepatic cooperation of non-transformed intestinal and hepatic cells are not available. The aim of our research is to establish a new porcine cell co-culture model constituted from differentiated intestinal epithelial cells (IPEC-J2) and primary hepatocytes, and to determine the changes in the expression levels of the xenobiotic transforming cytochrome P450 in porcine liver cells, as caused by pro-inflammatory mediator molecules produced by the intestinal epithelial cells, in response to microbial lipopolysaccharide (LPS) and reactive oxygen species. The expression of pro-inflammatory cytokines and cytochrome P450 iso-enzymes are determined at the level of transcription (qRT-PCR) and translation (ELISA and Western blot analysis). Thirdly, the potential anti-inflammatory activity of total spent culture supernatant of probiotic microbes (Lactobacillus and other bacterium species) and that of their single microbial products (such as, for instance, sodium-n-butyrate) will be investigated in the co-culture model.<\/p>","protected":false},"excerpt":{"rendered":"<p>Investigations with cell co-culture model systems are needed to understand the complex interrelationships between such processes as intestinal absorption, bioavailability and hepatic biotransformation in the animal organism. However, at present, suitable animal co-culture models mimicking the in vivo enterohepatic cooperation of non-transformed intestinal and hepatic cells are not available. The aim of our research is<\/p>\n","protected":false},"author":6022,"featured_media":0,"parent":873,"menu_order":154,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"categories":[],"tags":[],"class_list":["post-11103","page","type-page","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/univet.hu\/en\/wp-json\/wp\/v2\/pages\/11103","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/univet.hu\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/univet.hu\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/univet.hu\/en\/wp-json\/wp\/v2\/users\/6022"}],"replies":[{"embeddable":true,"href":"https:\/\/univet.hu\/en\/wp-json\/wp\/v2\/comments?post=11103"}],"version-history":[{"count":0,"href":"https:\/\/univet.hu\/en\/wp-json\/wp\/v2\/pages\/11103\/revisions"}],"up":[{"embeddable":true,"href":"https:\/\/univet.hu\/en\/wp-json\/wp\/v2\/pages\/873"}],"wp:attachment":[{"href":"https:\/\/univet.hu\/en\/wp-json\/wp\/v2\/media?parent=11103"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/univet.hu\/en\/wp-json\/wp\/v2\/categories?post=11103"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/univet.hu\/en\/wp-json\/wp\/v2\/tags?post=11103"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}