{"id":186856,"date":"2025-06-23T11:29:57","date_gmt":"2025-06-23T09:29:57","guid":{"rendered":"https:\/\/univet.hu\/education\/courses\/in-vitro-sejttechnologia\/"},"modified":"2025-06-23T12:50:48","modified_gmt":"2025-06-23T10:50:48","slug":"invitrocell","status":"publish","type":"page","link":"https:\/\/univet.hu\/en\/education\/courses\/invitrocell\/","title":{"rendered":"In vitro cell technology"},"content":{"rendered":"

The primary objective of the course is to introduce the applications of in vitro<\/em> cell lines in preclinical experiments and their associated limitations. In animal experiments, a fundamental requirement is to comply with the 3R rule system (Replacement, Reduction, Refinement), which requires researchers to use in vitro<\/em> methods instead of in vivo<\/em> experiments whenever possible. The use of in vitro<\/em> cell cultures offers this opportunity. The course provides students with a theoretical foundation for understanding the basic conditions required for maintaining and utilizing cell cultures. In addition to the limitations of in vivo<\/em> methods, the course material also covers the presentation of new innovative systems that can be useful for students during clinical research.<\/p>

The course is held in English. Due to the course’s structure, participation in the classes is recommended to complete the subject.<\/p>

Students give an account of their knowledge in a written tests and exam.<\/p>

Prequisite:<\/strong> Veterinary Physiology 1<\/p>

 <\/p>

<\/h3>

Course syllabus: (weekly breakdown of topics)<\/strong><\/p>
Semester week<\/strong><\/td> Topic<\/strong><\/td> <\/tr>
1. (2h)<\/td> Basics of in vitro<\/em> cell technology<\/td> <\/tr>
2. (2h)<\/td> Working in the aseptic area, basic equipment of cell culture laboratories<\/td> <\/tr>
3. (2h)<\/td> Isolation and maintenance of in vitro<\/em> cell cultures, main culture types<\/td> <\/tr>
4. (2h)<\/td> Solutions in the cell culture maintenance<\/td> <\/tr>
5. (2h)<\/td> 2D and 3D cell cultures, Spheroids and organoids<\/td> <\/tr>
6. (2h)<\/td> In vitro<\/em> plant cell cultures<\/td> <\/tr>
7. (2h)<\/td> Contamination in cellcultures, signs and avoidance<\/td> <\/tr>
8. (1h)<\/td> Exam<\/td> <\/tr> <\/tbody> <\/table>

 <\/p>","protected":false},"excerpt":{"rendered":"

The primary objective of the course is to introduce the applications of in vitro cell lines in preclinical experiments and their associated limitations. In animal experiments, a fundamental requirement is to comply with the 3R rule system (Replacement, Reduction, Refinement), which requires researchers to use in vitro methods instead of in vivo experiments whenever possible.<\/p>\n","protected":false},"author":3837,"featured_media":0,"parent":676,"menu_order":0,"comment_status":"open","ping_status":"open","template":"","meta":{"_acf_changed":false,"footnotes":""},"categories":[],"tags":[],"class_list":["post-186856","page","type-page","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/univet.hu\/en\/wp-json\/wp\/v2\/pages\/186856","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/univet.hu\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/univet.hu\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/univet.hu\/en\/wp-json\/wp\/v2\/users\/3837"}],"replies":[{"embeddable":true,"href":"https:\/\/univet.hu\/en\/wp-json\/wp\/v2\/comments?post=186856"}],"version-history":[{"count":5,"href":"https:\/\/univet.hu\/en\/wp-json\/wp\/v2\/pages\/186856\/revisions"}],"predecessor-version":[{"id":186891,"href":"https:\/\/univet.hu\/en\/wp-json\/wp\/v2\/pages\/186856\/revisions\/186891"}],"up":[{"embeddable":true,"href":"https:\/\/univet.hu\/en\/wp-json\/wp\/v2\/pages\/676"}],"wp:attachment":[{"href":"https:\/\/univet.hu\/en\/wp-json\/wp\/v2\/media?parent=186856"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/univet.hu\/en\/wp-json\/wp\/v2\/categories?post=186856"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/univet.hu\/en\/wp-json\/wp\/v2\/tags?post=186856"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}