{"id":3721,"date":"2018-04-05T19:40:00","date_gmt":"2018-04-05T17:40:00","guid":{"rendered":"https:\/\/univet.hu\/kat\/munkatarsak\/denes-lilla\/"},"modified":"2018-04-05T19:40:00","modified_gmt":"2018-04-05T17:40:00","slug":"denes-lilla","status":"publish","type":"page","link":"https:\/\/univet.hu\/kat\/munkatarsak\/denes-lilla\/","title":{"rendered":"D\u00e9nes Lilla"},"content":{"rendered":"

Bemutatkoz\u00e1s\u00a0 <\/span><\/b><\/p>

Egyetemi tanulm\u00e1nyaimat 2010-ben kezdtem a BME Biom\u00e9rn\u00f6k szak\u00e1n, a tudom\u00e1nyos munk\u00e1t 2013 nyar\u00e1n kezdtem az ELTE N\u00f6v\u00e9ny\u00e9lettani Tansz\u00e9k\u00e9n. Szakdolgozatomat amarant albumin feh\u00e9rj\u00e9k rizs modellrendszerben val\u00f3\u202f<\/span>in vitro<\/span><\/i>\u202fvizsg\u00e1lat\u00e1r\u00f3l \u00edrtam, diplomamunk\u00e1mban pedig tov\u00e1bbi\u202f<\/span>in vitro<\/span><\/i>\u202f\u00e9s\u202f<\/span>in vivo<\/span><\/i>\u202fk\u00eds\u00e9rletek is hozz\u00e1j\u00f6ttek.\u202f F\u0151 c\u00e9lunk egy olyan transzg\u00e9nikus rizs l\u00e9trehoz\u00e1sa volt, amely t\u00e1p\u00e9rt\u00e9ke magas, j\u00f3k a bel\u0151le k\u00e9sz\u00fclt t\u00e9szta dagaszt\u00e1si tulajdons\u00e1gai, \u00edgy j\u00f3 min\u0151s\u00e9g\u0171 glut\u00e9nmentes keny\u00e9r elk\u00e9sz\u00edt\u00e9s\u00e9hez is alkalmas. Ehhez egyr\u00e9szt mikrovalorigr\u00e1fos (mini dagaszt\u00f3g\u00e9p) k\u00eds\u00e9rletekkel bebizony\u00edtottuk, hogy egy magas esszenci\u00e1lis aminosav tartalm\u00fa feh\u00e9rje (amarant albumin) t\u00e9szt\u00e1ba val\u00f3 inkorpor\u00e1ci\u00f3ja pozit\u00edv hat\u00e1ssal van a t\u00e9szta reol\u00f3giai tulajdons\u00e1gaira. Tov\u00e1bbi s\u00fct\u00e9si k\u00eds\u00e9rletekkel meg\u00e1llap\u00edtottuk, hogy az amarant albuminokat tartalmaz\u00f3 t\u00e9szt\u00e1b\u00f3l jobb min\u0151s\u00e9g\u0171 keny\u00e9r s\u00fcthet\u0151, teh\u00e1t valamennyire siker\u00fclt p\u00f3tolnunk a rizsb\u0151l hi\u00e1nyz\u00f3 sik\u00e9r feh\u00e9rj\u00e9ket. Liofiliz\u00e1lt lisztmint\u00e1inkat SE-HPLC-vel vizsg\u00e1lva kider\u00fclt, hogy az oldhatatlan polimerek ar\u00e1nya is n\u0151tt az amarant albuminok hozz\u00e1ad\u00e1s\u00e1val, amely pozit\u00edv korrel\u00e1ci\u00f3ban van a t\u00e9szta min\u0151s\u00e9g\u00e9vel.\u202f Ezzel p\u00e1rhuzamosan l\u00e9trehoztunk egy olyan plazmid konstrukci\u00f3t, amely tartalmazta az AMA1 feh\u00e9rj\u00e9t k\u00f3dol\u00f3\u202f<\/span>ama1<\/span><\/i>\u202fg\u00e9nt, el\u0151tte egy endospermium specifikus prom\u00f3terrel, tov\u00e1bb\u00e1 k\u00e9t antibiotikum rezisztenci\u00e1t k\u00f3dol\u00f3\u202fg\u00e9nt (szelekci\u00f3s marker) \u00e9s az Agrobakt\u00e9rium-medi\u00e1lta transzform\u00e1ci\u00f3hoz sz\u00fcks\u00e9ges hat\u00e1rol\u00f3 szekvenci\u00e1kat. Ezt a konstrukci\u00f3t k\u00e9miai kompetens\u202f<\/span>Agrobacterium tumafaciens<\/span><\/i>\u202fsejtekbe transzform\u00e1ltuk, majd \u00e9rett rizs kalluszokat fert\u0151zt\u00fcnk. A konstrukci\u00f3nkra val\u00f3 szelekci\u00f3 ut\u00e1n a kalluszokb\u00f3l n\u00f6v\u00e9nyeket nevelt\u00fcnk, majd PCR m\u00f3dszerrel igazoltuk az\u202f<\/span>ama1<\/span><\/i>\u202fg\u00e9n rizs genomba val\u00f3 be\u00e9p\u00fcl\u00e9s\u00e9t.<\/span>\u00a0<\/span><\/p>

2016 nyar\u00e1n v\u00e9geztem a BME Biom\u00e9rn\u00f6k MSc k\u00e9pz\u00e9s\u00e9n \u00e9s kalandos \u00faton az \u00c1llatorvostudom\u00e1nyi Egyetem Patol\u00f3giai Tansz\u00e9k\u00e9n Balka Gyul\u00e1hoz ker\u00fcltem a PCR laborba, majd egy \u00e9vre r\u00e1 megkezdtem PhD tanulm\u00e1nyaimat.<\/span>\u00a0<\/span><\/p>

F\u0151bb kutat\u00e1si ter\u00fcletek:<\/span><\/b>\u00a0<\/span><\/p>

T\u00f6bbnyire \u00e1ltal\u00e1nos v\u00edrus diagnosztik\u00e1val \u00e9s PRRSV tipiz\u00e1l\u00e1s\u00e1val foglalkozunk, PhD t\u00e9m\u00e1m pedig egy \u00fajonnan azonos\u00edtott sert\u00e9s v\u00edrus,\u202faz atipikus sert\u00e9s-pestiv\u00edrussal (APPV)\u202fkapcsolatos kutat\u00e1saimat foglalja \u00f6ssze. Ez a v\u00edrus felel\u0151s r\u00e9szben a\u202fsert\u00e9sek eset\u00e9ben megfigyelhet\u0151\u202freszket\u0151k\u00f3r\u00e9rt, amely a fert\u0151z\u00f6tt koc\u00e1k ut\u00f3dainak fej\u00e9nek \u00e9s v\u00e9gtagjainak remeg\u00e9s\u00e9t okozza. Hab\u00e1r ez k\u00f6zvetlen\u00fcl nem okoz elhull\u00e1st, a reszket\u0151k\u00f3r megakad\u00e1lyozhatja a malacokat a t\u00e1pl\u00e1lkoz\u00e1sban, ami miatt n\u00f6veked\u00e9si visszamaradotts\u00e1g \u00e9s el\u00e9hez\u00e9s is fell\u00e9phet. M\u00e1jusra siker\u00fclt t\u00f6bb mint\u00e1ban is azonos\u00edtani a v\u00edrust \u00e9s r\u00e9szleges szekvenci\u00e1kat is kisajtolni bel\u0151l\u00fck, majd egy filogenetikai anal\u00edzis alapj\u00e1n m\u00e1r t\u00f6bb t\u00f6rzset is el tudtunk k\u00fcl\u00f6n\u00edteni. Kutat\u00e1som c\u00e9lja az\u202fAPPV hazai prevalenci\u00e1j\u00e1nak felm\u00e9r\u00e9se, a meghat\u00e1rozott szekvenci\u00e1k\u202ffilogenetikai elemz\u00e9se sor\u00e1n a v\u00edrus evol\u00faci\u00f3j\u00e1nak felt\u00e1r\u00e1sa, tov\u00e1bb\u00e1 a\u202fv\u00edrus sz\u00f6veti lokaliz\u00e1ci\u00f3j\u00e1nak\u202fvizsg\u00e1lata egy \u00fajabb RNS\u202fin situ<\/i>\u202fhibridiz\u00e1ci\u00f3s m\u00f3dszerrel (RNAscope).<\/p>

Ezeken fel\u00fcl kutat\u00f3csoportunk tagja egy nemzetk\u00f6zi konzorciumnak, amely Horizon 2020 t\u00e1mogat\u00e1ssal egy \u00faj, antig\u00e9n-antitest \u00e9s fel\u00fcleti plazmon rezonancia elv\u00e9n m\u0171k\u00f6d\u0151 diagnosztikai eszk\u00f6z l\u00e9trehoz\u00e1s\u00e1n dolgozott, amelynek c\u00e9lja a f\u0151bb kock\u00e1zati forr\u00e1st jelent\u0151 sert\u00e9s v\u00edrusok gyors \u00e9s on-site kimutat\u00e1sa.<\/span>\u00a0<\/span><\/p>

Kutat\u00f3csoportunk tagja egy Gent b\u00e1zis\u00fa konzorciumnak, amely c\u00e9lja nanopore \u00fajgener\u00e1ci\u00f3s szekven\u00e1l\u00f3 m\u00f3dszer haszn\u00e1lat\u00e1val felt\u00e9rk\u00e9pezni a sert\u00e9sekben megtal\u00e1lhat\u00f3 \u00f6sszes v\u00edrus \u00e9s bakt\u00e9rium teljes genomj\u00e1t.\u00a0<\/span>\u00a0<\/span><\/p>

El\u0151rehalad\u00e1s:<\/span><\/b>\u00a0<\/span><\/p>

\u202fEredm\u00e9nyeimet 2 k\u00fclf\u00f6ldi \u00e9s 3 magyarorsz\u00e1gi konferenci\u00e1n ismertettem, eddig 4 els\u0151szerz\u0151s cikkem jelent meg, ebb\u0151l 2 az APPV-vel kapcsolatos kutat\u00e1saimb\u00f3l, ezeken fel\u00fcl tov\u00e1bbi 8 cikkben vagyok t\u00e1rsszerz\u0151.\u00a0<\/span>\u00a0<\/span><\/p>

Eddigi szakdolgozat\/TDK hallgat\u00f3k sz\u00e1ma: 4<\/span><\/b>\u00a0<\/span><\/p>

Publik\u00e1ci\u00f3s lista (Top10)<\/span><\/b>\u00a0<\/span><\/p>

MTMT:\u202f <\/span>\u202f<\/span>\u00a0<\/span><\/p>
1.<\/td>

Denes, L<\/span><\/a>\u202f;\u202f<\/span>Horvath, DG*<\/span><\/a>\u202f;\u202fDuran, O\u202f;\u202fRatkhjen, PH\u202f;\u202fKraft, C\u202f;\u202f<\/span>Acs, B<\/span><\/a>\u202f;\u202f<\/span>Szasz, AM<\/span><\/a>\u202f;\u202fRumenapf, T\u202f;\u202f<\/span>Papp, M<\/span><\/a>\u202f;\u202fLadinig, A\u202fet al.<\/span>\u00a0<\/span><\/p>

In Situ Hybridization of PRRSV-1 Combined with Digital Image Analysis in Lung Tissues of Pigs Challenged with PRRSV-1<\/span><\/a>\u00a0<\/span><\/p>

VETERINARY SCIENCES\u202f8\u202f:\u202f10\u202fPaper: 235 , 11 p.\u202f(2021)<\/span>\u00a0<\/span><\/td> <\/tr>

2.<\/td>

Denes, L<\/span><\/a>\u202f;\u202fRuedas-Torres, I\u202f;\u202f<\/span>Szilasi, A<\/span><\/a>\u202f;\u202f<\/span>Balka, G <\/span>✉<\/span><\/a>\u00a0<\/span><\/p>

Detection and localization of atypical porcine pestivirus in the testicles of naturally infected, congenital tremor affected piglets<\/span><\/a>\u00a0<\/span><\/p>

TRANSBOUNDARY AND EMERGING DISEASES\u202fPaper: DOI10.1111\/tbed.14355\u202f(2021)<\/span>\u00a0<\/span><\/td> <\/tr>

3.<\/td>

D\u00e9nes, Lilla<\/span><\/a>\u202f;\u202f<\/span>Biksi, Imre<\/span><\/a>\u202f;\u202fAlbert, Mih\u00e1ly\u202f;\u202f<\/span>Szeredi, Levente<\/span><\/a>\u202f;\u202f<\/span>G Knapp, D\u00e1niel<\/span><\/a>\u202f;\u202f<\/span>Szilasi, Anna<\/span><\/a>\u202f;\u202f<\/span>B\u00e1lint, \u00c1d\u00e1m<\/span><\/a>\u202f;\u202f<\/span>Balka, Gyula<\/span><\/a>\u00a0<\/span><\/p>

Detection and phylogenetic characterization of atypical porcine pestivirus strains in Hungary<\/span><\/a>\u00a0<\/span><\/p>

TRANSBOUNDARY AND EMERGING DISEASES\u202f65\u202f:\u202f6\u202fpp. 2039-2042. , 4 p.\u202f(2018)<\/span>\u00a0<\/span><\/td> <\/tr>

4.<\/td>

D\u00e9nes, Lilla<\/span><\/a>\u202f;\u202f<\/span>Cs\u00e1gola, Attila<\/span><\/a>\u202f;\u202fSch\u00f6nhardt, Kitti\u202f;\u202fHalas, M\u00e1t\u00e9\u202f;\u202f<\/span>Solymosi, Norbert<\/span><\/a>\u202f;\u202f<\/span>Balka, Gyula<\/span><\/a>\u00a0<\/span><\/p>

First report of porcine parainfluenza virus 1 (species Porcine respirovirus 1) in Europe<\/span><\/a>\u00a0<\/span><\/p>

TRANSBOUNDARY AND EMERGING DISEASES\u202f00\u202fPaper: tbed.13869\u202f(2020)<\/span>\u00a0<\/span><\/td> <\/tr>

5.<\/td>

Balka, G<\/span><\/a>\u202f;\u202fPodgorska, K\u202f;\u202fBrar, MS\u202f;\u202fBalint, A\u202f;\u202fCadar, D\u202f;\u202fCeler, V\u202f;\u202f<\/span>Denes, L<\/span><\/a>\u202f;\u202fDirbakova, Z\u202f;\u202fJedryczko, A\u202f;\u202fMarton, L\u202fet al.<\/span>\u00a0<\/span><\/p>

Genetic diversity of PRRSV 1 in Central Eastern Europe in 1994-2014: origin and evolution of the virus in the region<\/span><\/a>\u00a0<\/span><\/p>

SCIENTIFIC REPORTS\u202f8\u202fPaper: 7811\u202f(2018)<\/span>\u00a0<\/span><\/td> <\/tr>

6.<\/td>

Horv\u00e1th, D\u00e1vid G\u00e9za\u202f;\u202fS\u00f3s, Endre\u202f;\u202f<\/span>D\u00e9nes, Lilla<\/span><\/a>\u202f;\u202f<\/span>D\u00e1n, \u00c1d\u00e1m<\/span><\/a>\u202f;\u202f<\/span>Biksi, Imre<\/span><\/a>\u202f;\u202f<\/span>Balka, Gyula<\/span><\/a>\u00a0<\/span><\/p>

Az elef\u00e1ntok endotheliotropikus herpeszv\u00edrusa : Irodalmi \u00f6sszefoglal\u00f3<\/span><\/a>\u00a0<\/span><\/p>

MAGYAR \u00c1LLATORVOSOK LAPJA\u202f142\u202f:\u202f5\u202fpp. 303-312. , 10 p.\u202f(2020)<\/span><\/td> <\/tr>

7.<\/td>

Szilasi, Anna<\/span><\/a>\u202f;\u202f<\/span>D\u00e9nes, Lilla<\/span><\/a>\u202f;\u202f<\/span>Krik\u00f3, Eszter<\/span><\/a>\u202f;\u202fMurray, Caoimhe\u202f;\u202f<\/span>M\u00e1ndoki, M\u00edra<\/span><\/a>\u202f;\u202f<\/span>Balka, Gyula<\/span><\/a>\u00a0<\/span><\/p>

Prevalence of feline leukaemia virus and feline immunodeficiency virus in domestic cats in Ireland<\/span><\/a>\u00a0<\/span><\/p>

ACTA VETERINARIA HUNGARICA\u202f68\u202f:\u202f4\u202fpp. 413-420. , 8 p.\u202f(2020)<\/span>\u00a0<\/span><\/td> <\/tr>

8.<\/td>

Szilasi, Anna <\/span>✉<\/span><\/a>\u202f;\u202f<\/span>Denes, Lilla<\/span><\/a>\u202f;\u202f<\/span>Jakab, Csaba<\/span><\/a>\u202f;\u202fErdelyi, Ildiko\u202f;\u202fResende, Talita\u202f;\u202fVannucci, Fabio\u202f;\u202f<\/span>Csomor, Judit<\/span><\/a>\u202f;\u202f<\/span>Mandoki, Mira<\/span><\/a>\u202f;\u202f<\/span>Balka, Gyula<\/span><\/a>\u00a0<\/span><\/p>

In situ hybridization of feline leukemia virus in a primary neural B-cell lymphoma<\/span><\/a>\u00a0<\/span><\/p>

JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION\u202f32\u202f:\u202f3\u202fpp. 454-457. , 4 p.\u202f(2020)<\/span>\u00a0<\/span><\/td> <\/tr>

9.<\/td>

Montagnese, Concetta\u202f;\u202fBarattini, Paolo\u202f;\u202fGiusti, Alessandro\u202f;\u202f<\/span>Balka, Gyula<\/span><\/a>\u202f;\u202fBruno, Ugo\u202f;\u202fBossis, Ioannis\u202f;\u202fGelasakis, Athanasios\u202f;\u202fBonasso, Matteo\u202f;\u202fPhilmis, Panayiotis\u202f;\u202f<\/span>D\u00e9nes, Lilla<\/span><\/a>\u202fet al.<\/span>\u00a0<\/span><\/p>

A Diagnostic Device for In-Situ Detection of Swine Viral Diseases: The SWINOSTICS Project<\/span><\/a>\u00a0<\/span><\/p>

SENSORS\u202f19\u202f:\u202f2\u202fPaper: 407\u202f(2019)<\/span>\u00a0<\/span><\/td> <\/tr>

10<\/td>

Szilasi, Anna<\/span><\/a>\u202f;\u202f<\/span>D\u00e9nes, Lilla<\/span><\/a>\u202f;\u202f<\/span>Balka, Gyula<\/span><\/a>\u00a0<\/span><\/p>

A macsk\u00e1k leukaemiav\u00edrusa (Feline Leukemia Virus, FeLV) Irodalmi \u00f6sszefoglal\u00f3<\/span><\/a>\u00a0<\/span><\/p>

MAGYAR \u00c1LLATORVOSOK LAPJA\u202f140\u202f:\u202f8\u202fpp. 457-472. , 16 p.\u202f(2018)<\/span>\u00a0<\/span><\/td> <\/tr> <\/tbody> <\/table>

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\u00a0<\/span><\/p>

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\u202f<\/span>\u00a0<\/span><\/p>

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\u00a0<\/span><\/p>

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\u202f<\/span>\u00a0<\/span><\/p>

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\u00a0<\/span><\/p>

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 <\/p>","protected":false},"excerpt":{"rendered":"

Bemutatkoz\u00e1s\u00a0 Egyetemi tanulm\u00e1nyaimat 2010-ben kezdtem a BME Biom\u00e9rn\u00f6k szak\u00e1n, a tudom\u00e1nyos munk\u00e1t 2013 nyar\u00e1n kezdtem az ELTE N\u00f6v\u00e9ny\u00e9lettani Tansz\u00e9k\u00e9n. Szakdolgozatomat amarant albumin feh\u00e9rj\u00e9k rizs modellrendszerben val\u00f3\u202fin vitro\u202fvizsg\u00e1lat\u00e1r\u00f3l \u00edrtam, diplomamunk\u00e1mban pedig tov\u00e1bbi\u202fin vitro\u202f\u00e9s\u202fin vivo\u202fk\u00eds\u00e9rletek is hozz\u00e1j\u00f6ttek.\u202f F\u0151 c\u00e9lunk egy olyan transzg\u00e9nikus rizs l\u00e9trehoz\u00e1sa volt, amely t\u00e1p\u00e9rt\u00e9ke magas, j\u00f3k a bel\u0151le k\u00e9sz\u00fclt t\u00e9szta dagaszt\u00e1si tulajdons\u00e1gai, \u00edgy j\u00f3<\/p>\n","protected":false},"author":0,"featured_media":0,"parent":858,"menu_order":210,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"categories":[],"tags":[],"class_list":["post-3721","page","type-page","status-publish","hentry"],"acf":[],"yoast_head":"\nD\u00e9nes Lilla - \u00c1llatorvostudom\u00e1nyi Egyetem Budapest<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/univet.hu\/kat\/munkatarsak\/denes-lilla\/\" \/>\n<meta property=\"og:locale\" content=\"hu_HU\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"D\u00e9nes Lilla - \u00c1llatorvostudom\u00e1nyi Egyetem Budapest\" \/>\n<meta property=\"og:description\" content=\"Bemutatkoz\u00e1s\u00a0 Egyetemi tanulm\u00e1nyaimat 2010-ben kezdtem a BME Biom\u00e9rn\u00f6k szak\u00e1n, a tudom\u00e1nyos munk\u00e1t 2013 nyar\u00e1n kezdtem az ELTE N\u00f6v\u00e9ny\u00e9lettani Tansz\u00e9k\u00e9n. 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