Professional carrier (short)

Tanulmányok

Schools, education:

1972-1977. University of Veterinary Science, Budapest

1977. Graduation with a summa cum laude designation

Tudományos szakterület/kutatási terület

Main research topics:

– establishment and characterization of primary cell cultures,

– investigation of the absorption, metabolism and action of short chain fatty acids (histone deacetylase inhibitors) in vitro and in vivo,

– proliferation, differentiation and apoptosis of cultured cells treated with histone deacetylase inhibitors, non-steroidal anti-inflammatory drugs and antioxidant molecules in tumor and non-tumor gastrointestinal cell cultures,

– prevention of cellular damage caused by oxidative stress and inflammation,

– synergistic effect of antibacterial molecules, retention of resistance in their combination investigated in enteropathogenic microorganisms,

– apoptosis and differentiation induced by butyrate and their correlation with different ROS in colon cancer cells,

– investigation of the effect of probiotics in prevention and treatment of bovine metritis,

– inhibition of inflammation caused by oxidative stress in enterocytes by probiotic Lactobacilli: pathomechanism and applicability in reared piglets as antibiotic substitutes,

– investigation of metabolism, toxicity and interactions of butyrate in primary culture of hepatocytes ,

– study on the effect of T2 toxin and Ochratoxin A, B on the cell division in vitro

– Na-n-butyrate-dependent PKC isoenzymes in colon cancer cell lines

– investigation in porcine enterocyte-hepatocyte co-culture model the ways of prevention of modified metabolism of xenobiotics caused by oxidative stress.

Academic carrier

1988. Candidate of Sciences CSc (veterinary medicine): „Biochemistry of keratinization and formation of hyperkeratosis in the rumen epithelium”
1989. Doctor of Sciences DSc (HungarianAcademy of Sciences, veterinary medicine)): „Rumen epithelial cell culture as a model to study the cell division, differentiation, transport, metabolism and bacterial adherence”

Activities at the university

Membership

 Awards

Tanulmányút, kutatási projekt

Research fellowships:

1988. University of Veterinary Medicine Hannover Institute of Physiology / DAAD scholarship (3 months): Metabolism, proliferation, differentiation and apoptosis of cultured rumen epithelial cells.
1989. Freie Universität, Berlin, Institute of Physiology / Humbold scholarship (1 year): Metabolism, proliferation and transport processes of cultured rumen epithelial cells.

1987- short study tours (1-2 weeks), scientific research in the frame of several bilateral cooperations:

-Rowett Research Institute, Aberdeen (1992-1997, 8 study tours): Adhesion of plant and bacterial lectines to cultured rumen epithelial cells (English-Hungarian TÉT),

-Faculty of Medicine University of Innsbruck (1998-, 14 study tours): Investigation of short chain fatty acids (histone deacetylase inhibitors), non-steroidal anti-inflammatory drugs, polyphenol antioxidants in cell cultures in vitro (Austrian-Hungarian-French TÉT). Investigation of PKC isoenzymes in cell cultures, detection of apoptosistása (Osztrák- Magyar TÉT)

-Nippon Veterinary and Life Science University, Tokyo (2006), organization of bilateral research cooperation.

2008. Institute of Geotechnics of the Slovak Academy of Sciences, Košice: study ont he adhesion of probiotic enterococcus

Research projects

Principal investigator:

1. Gálfi P., Neogrády Zs., Kutas F., Veresegyházy T., Rimanóczy Á.: Biochemical characterization of rumen epithelial cells, their role in transport processes. OTKA (1254) project, 1991-1994.
2. Gálfi P., Neogrády Zs.: Competitive and interactive effects of plant lectines amd bacterial adhesines on the rumen epithelium. OTKA (016488) project, 1995-1997.
3. Gálfi P., Neogrády Zs., Rudas P.: Effect of short chain fatty acids and modification of H+ ion concentration on the adaptive processes of rumen mucosa. OTKA (26060) project, 1998-2001.
4. Gálfi P.: Additional project for OTKA (26060) project, 2001.
5. Gálfi P., Neogrády Zs., Rimanóczy Á.: Role of short chain fatty acids and certain arachidonate metabolites in the proliferation and apoptosis regulation of gastrointestinal cells. OTKA (T037401) project, 2002-2005.
6. Gálfi P.: Investigation of the effect of different feed supplements on the functional state of the gastrointestinal tract. (R+D project:.1992, 1993, 1994, 1996).
7. Gálfi P: NKB projects NKB-2000, NKB-2002
8. Gálfi P., Neogrády Zs.: Der Wirkungsmechanismus von Butyrat in gastrointestinalen Epithelzellen. Austrian-Hungarian Action Foundation 32ÖU17 (1998).
9. Gálfi P., Neogrády Zs.: Study on the apoptosis and inhibition of cell division after butyrate treatment of tumor cell lines and gastrointestinal cells. Hungarian-Austrian TÉT Project (A-10/1999), 2000-2001.
10. Gálfi P., Neogrády Zs.: Resveratrol induved cell proliferation inhibition in gastrointestinal tumor cells in correlation wizh eicosanoid metabolism. Hungarian-Austrian TÉT Project (A-18/01), 2002-2003.
11. Gálfi P. (consortium member): The reason of non-appropriate food safety in Hungary: methods of improvement. NKFP4, 2002-2003.
12. Gálfi P. (consortium member): Production of probiotic vegetable juices to improve food safety and human health, GVOP-3.1.1-2004-05-0076/3.0, 2005-2006.
13. Gálfi P., Neogrády Zs.: Selective effects of polyphenols on the apoptosis in tumor cells. Synergic effect of polyphenols with non-steroidal anti-inflammatory drugs and histone deacetylase inhibitors. Hungarian-Austrian TÉT Project (A-7/04), 2005-2006.
14. Gálfi P., Neogrády Zs.: Dietary polyphenols: Selectivity of cell death induction in tumor cells.Synergism with nonsteroidal anti-inflammatory drugs and histone deacetylase inhibitors. Hungarian-French TÉT Project (F-35/04), 2005-2007.
15. Gálfi P. Inhibition of inflammation caused by oxidative stress in enterocytes by probiotic Lactobacilli. (2007) NKB
16. Gálfi P. Investigation of the effect of probiotics in prevention and treatment of bovine metritis. (2008, 2009) NKB.
17. Gálfi P.: Study on the effect of T2 toxin and Ochratoxin A, B on the cell division in vitr.o NKB pályázat 2009.
18. Gálfi P.: Comparison of the growth of porcine intestinal cells cultured either on polycarbonate or collagene-coated inserts. NKB project 2010.
19. Gálfi P., Lehel J., Csizinszky R., Nagy G., Szekér K., Jerzsele Á., Kővágó Cs., Csikó Gy. Inhibition of inflammation caused by oxidative stress in enterocytes by probiotic Lactobacilli: pathomechanism and applicability in reared piglets as antibiotic substitutes.  OTKA-76133, 2009-2011.
20. Gálfi P., Jerzsele Á., Mátis G., Csikó Gy., Kővágó Cs., Szekér K., Csizinszky R., Kenéz Á., Neogrády Zs., Pásztiné Gere E., Palócz O. : Investigation the ways of prevention of modified metabolism of xenobiotics caused by oxidative stress in porcine enterocyte-hepatocyte co-culture models. OTKA-100701, 2012-2014.
21. Gálfi P., Csikó György, Farkas Orsolya, Jerzsele Ákos, Kővágó Csaba, Nagy Gábor, Palócz Orsolya, Pásztiné Gere Erzsébet: Molecular Pharmacological Research Group. KK-KF, 2013.
22. Gálfi P., Csikó György, Farkas Orsolya, Jerzsele Ákos, Kővágó Csaba, Nagy Gábor, Palócz Orsolya, Pásztiné Gere Erzsébet: Molecular Pharmacological Research Group. KK-KF, 2015.

Senior investigator:

1. Kutas F., Gálfi P., Neogrády Zs., Veresegyházy T., Rimanóczy Á.: Regulation of energy homeostasis in ruminants. OTKA (1538) project, 1986-1991.
2. Bokori J., Szigeti G., Gálfi P., Fekete S.: Butyrate as feed supplement in monogastric animals. OMFB (92-97-16-0207) project, 1992-1994.
3. Neogrády Zs., Gálfi P.: Investigation of the interactive effects of plant and microbial lectins on the physiological state and function of the rumen epithelium for improving the quality of beef produced for human consumption. EU COST project 909 (6905), 1993-1995.
4. Neogrády Zs., Gálfi P.: Effects of plant and bacterial lectin binding on the physiological state and function of the rumen epithelium. OMFB-BC TET project, 1994-1995.
5. Neogrády Zs., Gálfi P.: Role of ruminal epithelium in the bacterial adhesion of non-ruminant species. OTKA (02001) pályázat, 1996-1998.
6. Neogrády, Zs., Gálfi, P.: Study of the plant and bacterial lectin of the cultured rumen epithelial cells. MÖB-BC project, 1996-1998.
7. Rudas P. principal investigator, FKFP 0672/97 project, 1997-2001.
8. Neogrády Zs., Gálfi P.: NKB projects. Investigation of the interactive effects of plant and microbial lectins on the physiological state and function of the rumen epithelium. 1997, 1998, 1999, 2001
9. Bartha T., Gálfi P., Rónai Zs., Györffy A. OTKA T046914: Investigation of certain endocrine factors regulating energy homeostasis of domestic animals on molecular level. 2004-2007.
10. Frenyó Vilmos L., Gálfi P., Huszenicza Gy., Jancsik V., Sótonyi P., Halasy K., Bartha T., Kacskovics I., Neogrády Zs. OTKA TS49756: „Regulatory mechanisms in basic veterinary science: from molecules to organ systems”, 2005-2008.
11. Jerzsele Á., Gyetvai B. Pásztiné Gere E.: Optimized in vitro model for studying the effects of antimicrobial agents and feed additives in Lawsonia intracellularis in vitro artifical infection, NKB pályázat 2013.
12. Development of intelligent specialization: Cooperation of the University of Veterinary Medicine and Széchenyi István University. EFOP-3.6.1-16

Consulent: 1 student for thesis writing

Supervisor: 12 students in the frame of Students’ Research Circle. Placing at the Hungarian Conference of Students’ Research Circle: 2 first prizes, 1 second prize, 1 third prize.

Consulent: 4 PhD students

Supervisors: 4 PhD students (finished: Domokos Mónika, 2011, Jerzsele Ákos, 2009, Pásztiné Gere Erzsébet, 2013, Gyetvai Béla, 2019) and 2 PhD students (not yet finished).

Top ten publications (Top10)

MTMT: https://m2.mtmt.hu/gui2/?type=authors&mode=browse&sel=authors10002267