Academic year 2017/2018, fall semester
- Type of course and credits given: elective „B”, 2 credits
- Time: Thursday, 16:15-17:45
- Venue: „Kemény Armand” lecture hall (Department of Physiology and Biochemistry)
- Exam: written test
Topics and schedules of the lectures:
Date | Topic | Lecturer |
---|---|---|
28 September | Introduction. Overview of the basics of molecular biology. Tools of the recombinant gene technology: cloning and expression vectors, restriction endonucleases, DNA ligase, reverse transkriptase. | Kulcsár Anna |
5 October | The complete process of the recombinant gene technology (gene transfer, cloning): gene isolation, preparation of the recombinant DNA, identification of the transformed host cells with specific markers, production of recombinant proteins. | Dr. Mátis Gábor |
12 October | Practical applications of the recombinant gene technology in veterinary and human medicine. Possibilities in the regulation of gene expression. Epigenetic regulatory mechanisms. | Kulcsár Anna |
19 October | Examination methods in the molecular biology I.: PCR, nucleotide sequencing. | Prof. Harrach Balázs |
26 October | Examination methods in the molecular biology II.: qRT-PCR, Western blot. | Kulcsár Anna |
2 November | Introduction to the „omics” approach and system biology: genomics, transcriptomics, proteomics, metabolomics. New generation examination methods in the molecular biology. | Dr. Hatala Patrícia |
9 November | Cell cultures and their application in the molecular biology. | Dr. Mátis Gábor |
16 November | Written exam |
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