Course description

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Lectures theme

Academic year 2023/2024, spring semester

Type of course and credits given: elective „B”, 2 credits

Data: Tuesday, 17:15 – 18:45

Venue: online, TEAMS

Exam: written exam

Topics and schedules of the lectures:

	Topic	Lecturer
13 February	Introduction. Overview of the basics of molecular biology. Tools of the recombinant gene technology: cloning and expression vectors, restriction endonucleases, DNA ligase, reverse transkriptase.	Dr. Papp-Sebők Csilla
20 February	The complete process of the recombinant gene technology (gene transfer, cloning): gene isolation, preparation of the recombinant DNA, identification of the transformed host cells with specific markers, production of recombinant proteins.	Dr. Mátis Gábor
27 February	Practical applications of the recombinant gene technology in veterinary and human medicine. Possibilities in the regulation of gene expression. Epigenetic regulatory mechanisms.	Dr. Tráj Patrik
5 March	Examination methods in the molecular biology: Western blotting, ELISA.	Dr. Mackei Máté
12 March	Cell cultures and their application in the molecular biology I.: theoretical basics.	Dr. Márton Rege Anna
19 March	Cell cultures and their application in the molecular biology II.: practical research applications.	Dr. Papp-Sebők Csilla
26 March	EASTER HOLIDAY, NO LECTURE
2 April	Introduction to the „omics” approach and system biology: genomics, transcriptomics, proteomics, metabolomics. New generation examination methods in the molecular biology.	Dr. Papp Márton
9 April	NO LECTURE
16 April	Written exam (Unipoll, időpont: 18:00-22:00)

Further information: Matis.Gabor@univet.hu