Default view
Education Courses Gene technology

Gene technology

Language
English
Nature
optional B
Method of evaluation
semi-final examination
Specialization
állatorvos (magyar)
Allow for

Course description

Lectures theme

Academic year 2021/2022, spring semester

Type of course and credits given: elective „B”, 2 credits

Data: Friday, 13:15 – 15:00

Venue: Armand Kemény lecture hall

Exam: written

 

Topics and schedules of the lectures:

Topic Lecturer
18 February Introduction. Overview of the basics of molecular biology. Tools of the recombinant gene technology: cloning and expression vectors, restriction endonucleases, DNA ligase, reverse transkriptase. Dr. Mátis Gábor
25 February The complete process of the recombinant gene technology (gene transfer, cloning): gene isolation, preparation of the recombinant DNA, identification of the transformed host cells with specific markers, production of recombinant proteins. Dr. Mátis Gábor
4 March Practical applications of the recombinant gene technology in veterinary and human medicine. Possibilities in the regulation of gene expression. Epigenetic regulatory mechanisms. Dr. Tráj Patrik
11 March Examination methods in the molecular biology: Western blotting, ELISA. Dr. Mackei Máté
18 March Cell cultures and their application in the molecular biology I. – theoretical basics Dr. Mackei Máté
25 March Cell cultures and their application in the molecular biology II. – practical applications Dr. Papp-Sebők Csilla
1 April Introduction to the „omics” approach and system biology: genomics, transcriptomics, proteomics, metabolomics. New generation examination methods in the molecular biology. Dr. Papp Márton
8 April Written exam  

 

Further information: Matis.Gabor@univet.hu