Language
English
Nature
optional B
Method of evaluation
semi-final examination
Specialization
állatorvos (magyar)
Department
Department of Physiology and Biochemistry
Allow for
Course description
Lectures theme
Academic year 2021/2022, spring semester
Type of course and credits given: elective „B”, 2 credits
Data: Friday, 13:15 – 15:00
Venue: Armand Kemény lecture hall
Exam: written
Topics and schedules of the lectures:
| Topic | Lecturer | |
| 18 February | Introduction. Overview of the basics of molecular biology. Tools of the recombinant gene technology: cloning and expression vectors, restriction endonucleases, DNA ligase, reverse transkriptase. | Dr. Mátis Gábor |
| 25 February | The complete process of the recombinant gene technology (gene transfer, cloning): gene isolation, preparation of the recombinant DNA, identification of the transformed host cells with specific markers, production of recombinant proteins. | Dr. Mátis Gábor |
| 4 March | Practical applications of the recombinant gene technology in veterinary and human medicine. Possibilities in the regulation of gene expression. Epigenetic regulatory mechanisms. | Dr. Tráj Patrik |
| 11 March | Examination methods in the molecular biology: Western blotting, ELISA. | Dr. Mackei Máté |
| 18 March | Cell cultures and their application in the molecular biology I. – theoretical basics | Dr. Mackei Máté |
| 25 March | Cell cultures and their application in the molecular biology II. – practical applications | Dr. Papp-Sebők Csilla |
| 1 April | Introduction to the „omics” approach and system biology: genomics, transcriptomics, proteomics, metabolomics. New generation examination methods in the molecular biology. | Dr. Papp Márton |
| 8 April | Written exam |
Further information: Matis.Gabor@univet.hu