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Education Courses Gene technology

Gene technology

Language
English
Nature
optional B
Method of evaluation
semi-final examination
Specialization
állatorvos (magyar)
Allow for

Course description

Lectures theme

Academic year 2023/2024, spring semester

Type of course and credits given: elective „B”, 2 credits

Data: Tuesday, 17:15 – 18:45

Venue: online, TEAMS

Exam: written exam

Topics and schedules of the lectures:

Topic Lecturer
13 February Introduction. Overview of the basics of molecular biology. Tools of the recombinant gene technology: cloning and expression vectors, restriction endonucleases, DNA ligase, reverse transkriptase. Dr. Papp-Sebők Csilla
20 February The complete process of the recombinant gene technology (gene transfer, cloning): gene isolation, preparation of the recombinant DNA, identification of the transformed host cells with specific markers, production of recombinant proteins. Dr. Mátis Gábor
27 February Practical applications of the recombinant gene technology in veterinary and human medicine. Possibilities in the regulation of gene expression. Epigenetic regulatory mechanisms. Dr. Tráj Patrik
5 March Examination methods in the molecular biology: Western blotting, ELISA. Dr. Mackei Máté
12 March Cell cultures and their application in the molecular biology I.: theoretical basics. Dr. Márton Rege Anna
19 March Cell cultures and their application in the molecular biology II.: practical research applications. Dr. Papp-Sebők Csilla
26 March EASTER HOLIDAY, NO LECTURE
2 April Introduction to the „omics” approach and system biology: genomics, transcriptomics, proteomics, metabolomics. New generation examination methods in the molecular biology. Dr. Papp Márton
9 April NO LECTURE
16 April Written exam (Unipoll, időpont: 18:00-22:00)

Further information: Matis.Gabor@univet.hu