English
fakultatív C
vizsga (kollokvium)
2
15
veterinary (English)
Department of Physiology and Biochemistry
- Vet EN
Course description
The primary objective of the course is to introduce the applications of in vitro cell lines in preclinical experiments and their associated limitations. In animal experiments, a fundamental requirement is to comply with the 3R rule system (Replacement, Reduction, Refinement), which requires researchers to use in vitro methods instead of in vivo experiments whenever possible. The use of in vitro cell cultures offers this opportunity. The course provides students with a theoretical foundation for understanding the basic conditions required for maintaining and utilizing cell cultures. In addition to the limitations of in vivo methods, the course material also covers the presentation of new innovative systems that can be useful for students during clinical research.
The course is held in English. Due to the course’s structure, participation in the classes is recommended to complete the subject.
Students give an account of their knowledge in a written tests and exam.
Prequisite: Veterinary Physiology 1
Course syllabus: (weekly breakdown of topics)
| Semester week | Topic |
| 1. (2h) | Basics of in vitro cell technology |
| 2. (2h) | Working in the aseptic area, basic equipment of cell culture laboratories |
| 3. (2h) | Isolation and maintenance of in vitro cell cultures, main culture types |
| 4. (2h) | Solutions in the cell culture maintenance |
| 5. (2h) | 2D and 3D cell cultures, Spheroids and organoids |
| 6. (2h) | In vitro plant cell cultures |
| 7. (2h) | Contamination in cellcultures, signs and avoidance |
| 8. (1h) | Exam |