Homeostasis in the animal organism, transport processes across the biological membrane, characterization, and regulation of the enzymatic reactions. Basic knowledge of molecular biology.
Important note: All the PPT presentations are available on the Moodle page of the subject.
|16 September||Introduction. Internal environment, homeostasis. Isovolaemia, isotonia, isoionia. Isohydria, buffer systems. Structure of the biological membranes.||Dr. Neogrády Zsuzsanna|
|23 September||Transport across the biological membranes. Classification and structure of the proteinogenic amino acids. The peptide bond.||Dr. Mackei Máté|
|30 September||General characterization and structure of proteins. Denaturation and renaturation of proteins. Classification of proteins. Structural proteins: collagen, elastin, keratin.||Dr. Mackei Máté|
|7 October||Characterization of enzymes. Mechanism of enzyme action. Reversibility of enzymatic reactions. Factors influencing the velocity of enzymatic reactions. The regulation of the enzyme activity. The inhibition of the enzyme activity. Nomenclature, classification and cellular localization of enzymes.||Dr. Mackei Máté|
|14 October||Structure of nucleotides. De novo synthesis and degradation of purine nucleotides.||Dr. Neogrády Zsuzsanna|
|21 October||De novo synthesis and degradation of pyrimidine nucleotides. Salvage pathways of nucleotide metabolism (resynthesis of nucleotides). Metabolism of deoxyribonucleotides. The structure and function of DNA.||Dr. Neogrády Zsuzsanna|
|28 October||Replication of DNA in prokaryotes and eukaryotes. Mutations, repair mechanisms. The structure of the transcription unit in prokaryotes and eukaryotes. Transcription and its regulation in prokaryotes.||Dr. Mackei Máté|
|4 November||Transcription and its regulation in eukaryotes: gene expression, epigenetic regulatory mechanisms. Structure of the ribosomes. Activation of the amino acids. The translation in eukaryotes.||Dr. Mackei Máté|
|11 November||The translation in prokaryotes. Posttranslational modifications of proteins. Recombinant DNA technology and biotechnology: aims, tools, steps and application possibilities.||Dr. Mackei Máté|
|18 November||General introduction to the intermediary metabolism. Biological oxidation. Conservation of metabolic energy in the cell: high energy phosphates. Metabolism of creatine. Chemistry of carbohydrates: role, classification: monosaccharides, oligosaccharides, homo- and heteropolysaccharides.||Dr. Mackei Máté|
|25 November||Cori cycle. Glycogen metabolism: glycogenolysis and glycogenesis. The glucagon and insulin signalling pathways. Glycolysis and gluconeogenesis: steps.||Dr. Neogrády Zsuzsanna|
|2 December||Glycolysis and gluconeogenesis: regulation, localization. Glycerol phosphate shuttle. Pasteur effect. The oxidation of pyruvate to acetyl CoA. The citric acid cycle.||Dr. Neogrády Zsuzsanna|
|9 December||The respiratory chain and the oxidative phosphorylation. The pentose phosphate pathway.||Dr. Neogrády Zsuzsanna|
|16 December||Blood sugar level and its regulation. Metabolism of fructose and galactose.||Dr. Neogrády Zsuzsanna|
Practical lessons theme
Students are hereby informed that the order and dates of the laboratory practicals in Veterinary Biochemistry 1. are as follows:
|I.||Blood plasma||20-24 September
27 September -1 October
|III.||General properties of enzymes||18-22 October
|IV.||Digestive enzymes||8-12 November
|V.||Nucleic acids||22-26 November
29 November -3 December
Practicals III-V. : 6th Dec (Monday), starting is possible between 1200 – 1400 o’clock.
Practicals I., II. : 7th Dec (Tuesday), starting is possible between 1200 – 1400 o’clock.
Dr. Papp-Sebők Csilla, professor assistant (E-mail: Sebok.Csilla@univet.hu) is responsible for the biochemical labs in English. For lab change requests and further information please contact: Sebok.Csilla@univet.hu.
Regarding lab safety, it is obligatory for every student to read and watch the provided narrated PPT files, which can be found in the Moodle system.
Students are hereby informed that the prevalent qualification system and requirements in subject “Veterinary Biochemistry 1.” for the fall semester of the academic year 2021/2022 are as follows:
- Regular personal or online visiting of the lectures
- Successful performance of all laboratory practicals
- Completion of 60% of the maximal scores in the laboratory practicals
- Completion of 60% of the maximal scores on the written midterm test
The complete qualification system of the subject can be downloaded from the webpage of the Division of Biochemistry: https://univet.hu/en/biochemvet/.
The date of the midterm test: 10th November 2021 at 715 am. in the „Kemény Armand” lecture hall.
Retake: 24th November 2021 at 715 am. in the „Kemény Armand” lecture hall.
Extra retake (from whole Biochemistry I): 17th December 2021 at 715 am. in the Kemény Armand lecture hall
The date of the Biochemistry 2. midterm test: 1th December 2021 at 715 am (Please write an e-mail before the Biochemistry 2. midterm because of application and confirmation)
Consultation possibility: online (E-mail: Neogrady.Zsuzsanna@univet.hu)