Homeostasis in the animal organism, transport processes across the biological membrane, characterization and regulation of the enzymatic reactions. Basic knowledge of the molecular biology.
Students are hereby informed that the topics of the VETERINARY Biochemistry 1. lectures in the fall semester of the academic year 2020/2021 are the followings:
|Week 1||Introduction. Internal environment, homeostasis. Isovolaemia, isotonia, isoionia. Isohydria, buffer systems. Structure of the biological membranes.||Dr. Neogrády Zsuzsanna|
|Week 2||Transport across the biological membranes. Classification and structure of the proteinogenic amino acids. The peptide bond.||Dr. Neogrády Zsuzsanna|
|Week 3||General characterization and structure of proteins. Denaturation and renaturation of proteins. Classification of proteins. Structural proteins: collagen, elastin, keratin.||Dr. Neogrády Zsuzsanna|
|Week 4||Characterization of enzymes. Mechanism of enzyme action. Reversibility of enzymatic reactions. Factors influencing the velocity of enzymatic reactions. The regulation of the enzyme activity. The inhibition of the enzyme activity. Nomenclature, classification and cellular localization of enzymes.||Dr. Neogrády Zsuzsanna|
|Week 5||Structure of nucleotides. De novo synthesis and degradation of purine nucleotides.||Dr. Neogrády Zsuzsanna|
|Week 6||De novo synthesis and degradation of pyrimidine nucleotides. Salvage pathways of nucleotide metabolism (resynthesis of nucleotides). Metabolism of deoxyribonucleotides. The structure and function of DNA.||Dr. Neogrády Zsuzsanna|
|Week 7||Replication of DNA in prokaryotes and eukaryotes. Mutations, repair mechanisms. The structure of the transcription unit in prokaryotes and eukaryotes. Transcription and its regulation in prokaryotes.||Dr. Mackei Máté|
|Week 8||Transcription and its regulation in eukaryotes: gene expression, epigenetic regulatory mechanisms. Structure of the ribosomes. Activation of the amino acids. The translation in eukaryotes.||Dr. Mackei Máté|
|Week 9||The translation in prokaryotes. Posttranslational modifications of proteins. Recombinant DNA technology and biotechnology: aims, tools, steps and application possibilities.||Dr. Mackei Máté|
|Week 10||General introduction to the intermediary metabolism. Biological oxidation. Conservation of metabolic energy in the cell: high energy phosphates. Metabolism of creatine. Chemistry of carbohydrates: role, classification: monosaccharides, oligosaccharides, homo- and heteropolysaccharides.||Dr. Neogrády Zsuzsanna|
|Week 11||Cori cycle. Glycogen metabolism: glycogenolysis and glycogenesis. The glucagon and insulin signalling pathways. Glycolysis and gluconeogenesis: steps.||Dr. Neogrády Zsuzsanna|
|Week 12||Glycolysis and gluconeogenesis: regulation, localization. Glycerol phosphate shuttle. Pasteur effect. The oxidation of pyruvate to acetyl CoA. The citric acid cycle.||Dr. Neogrády Zsuzsanna|
|Week 13||The respiratory chain and the oxidative phosphorylation. The pentose phosphate pathway.||Dr. Neogrády Zsuzsanna|
|Week 14||Blood sugar level and its regulation. Metabolism of fructose and galactose.||Dr. Neogrády Zsuzsanna|
Practical lessons theme
Students are hereby informed that the order and dates of the laboratory practicals in Veterinary Biochemistry 1. are as follows:
|I.||Blood plasma||21-25 September
28 September -2 October
|III.||General properties of enzymes||19-22 October
|IV.||Digestive enzymes||2-6 November
|V.||Nucleic acids||16-20 November
Practicals III-V.: 30th Nov (Monday), starting is possible between 1200 – 1400 o’clock.
Practicals I., II.: 1st Dec (Tuesday), starting is possible between 1200 – 1400 o’clock.
Dr. Sebők Csilla, professor assistant (E-mail: Sebok.Csilla@univet.hu) is responsible for the biochemical labs in English. For lab change request and further information please contact: Sebok.Csilla@univet.hu.
Veterinary Biochemistry laboratory practical regulations
Fall semester, academic year 2020/2021
Veterinary Biochemistry laboratory practicals in the fall semester of academic year 2020/2021, considering the pandemic situation, until further regulations or revocation, for the safety of our students and our staff members, will be held as follows:
- The relevant chapters of the laboratory manual will be available on Moodle server of the university. Please download them, and as they also function as a report for the laboratory practical, bring a printed, or an editable, fillable electronic version to the lab practical. The textbook is also available for purchase in the bookshop of the campus.
- The first introductory part of the labs (overview of the theoretical background of the topic, technical advices, demonstration experiments) will be recorded prior to the practical, and will be uploaded to Moodle as video files. The individual processing of the aforementioned recordings is a compulsory part of the laboratory course. During the in person laboratory practicals only certain manual experiments are to be carried out, the preparation shall be done individually at home.
- For each laboratory practical, the relevant chapter of the manual and the instructions given in the video recordings have to be learned thoroughly. The manual has to be filled in beforehand, except those gaps (observations and results) which you have to fill in during practical. Please also read through the relevant notes given during the theoretical lectures.
- In order to keep the appropriate physical distancing in the laboratory, the maximum number of participants is limited to 9 persons on each practical. Therefore, the groups will be divided into two subgroups (e.g.: 1/A and 1/B subgroups). The final schedule will be available (downloadable) on the first week of the semester. In accordance, the practical will be held at the time given in the timetable for the first subgroup (i.e.: subgroup 1/A), and 90 minutes later for the second half of the group (i.e.: subgroup 1/B).
- You are allowed to take part in your designated group’s practical exclusively, and only because of particular reason you can participate in another group’s practical, with the previous approval from Dr. Csilla Sebők. You can ask for permission via e-mail (firstname.lastname@example.org), until the working day before the wanted lab till 14:00. In the e-mail please state the name of the demonstrator and the scheduled start of practical chosen.
- You have to wear appropriate face covering or face mask, rubber gloves and white lab coat at all times during practical, please bring them with you. All through the duration of the lab, upon entry and the exit you must keep 1.5 m physical distance from one another. You can only take the lab coat, the printed version of the laboratory manual and your pen to the lab bench and must leave everything else in the cupboard of the lab.
- At the beginning of each lab you have to answer 5 multiple choice questions in 5 minutes. (Except on the 5th lab at which you have to successfully answer 3 test questions and draw the given molecule’s structure.) The topic of the multiple choice test will cover the currently given chapter of the manual.
- After the successful test you will have to carry out certain experiments (you will be informed in forward which ones) with the help of the demonstrator and by the instructions given in the video recording. After completion the given experiment, please fill in the observations and the results in the manual. The face-to-face section of the practical – due to the division of groups – is going to be a maximum of 70 minutes.
- After the completion of each experiment you will have to hand in the manual to the demonstrator, and your points will be determined based on the entry test, the quality of your experiments and your observations and results written in the manual (0-5 points). (Due to safety precautions oral tests at the end of the labs will not be held.) You have to achieve the minimum of 2 points for the practical to be successful. The semester will be accepted, if you reached at least 15 points during the 5 practicals.
Budapest, 14. 09. 2020
Students are hereby informed that the prevalent qualification system and requirements in subject “Veterinary Biochemistry 1.” for the fall semester of the academic year 2020/2021 are as follows:
- Regular personal or online visiting of the lectures
- Successful performance of all laboratory practicals
- Completion of 60% of the maximal scores in the laboratory practicals
- Completion of 60% of the maximal scores on the written midterm test
The complete qualification system of the subject can be downloaded from the webpage of the Division of Biochemistry: https://univet.hu/en/biochemvet/.
The date of the midterm test: 4th November 2020 at 715 am. in the „Kemény Armand” lecture hall.
Retake: 18th November 2020 at 715 am. in the „Kemény Armand” lecture hall.
Extra retake (from whole Biochemistry I): 18th December 2020 at 715 am. in the Kemény Armand lecture hall
Consultation possibility: online (E-mail: Neogrady.Zsuzsanna@univet.hu)