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Gene technology

Academic year 2017/2018, fall semester

  • Type of course and credits given: elective „B”, 2 credits
  • Time: Thursday, 16:15-17:45
  • Venue: „Kemény Armand” lecture hall (Department of Physiology and Biochemistry)
  • Exam: written test

Lecture slide downloads »

Topics and schedules of the lectures:

Date Topic Lecturer
28 September Introduction. Overview of the basics of molecular biology. Tools of the recombinant gene technology: cloning and expression vectors, restriction endonucleases, DNA ligase, reverse transkriptase. Kulcsár Anna
5 October The complete process of the recombinant gene technology (gene transfer, cloning): gene isolation, preparation of the recombinant DNA, identification of the transformed host cells with specific markers, production of recombinant proteins. Dr. Mátis Gábor
12 October Practical applications of the recombinant gene technology in veterinary and human medicine. Possibilities in the regulation of gene expression. Epigenetic regulatory mechanisms. Kulcsár Anna
19 October Examination methods in the molecular biology I.: PCR, nucleotide sequencing. Prof. Harrach Balázs
26 October Examination methods in the molecular biology II.: qRT-PCR, Western blot. Kulcsár Anna
2 November Introduction to the „omics” approach and system biology: genomics, transcriptomics, proteomics, metabolomics. New generation examination methods in the molecular biology. Dr. Hatala Patrícia
9 November Cell cultures and their application in the molecular biology. Dr. Mátis Gábor
16 November Written exam

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