- Vet EN
Denomination: Veterinary Virology
Department: Department of Microbiology and Infectious Diseases
Scheduled place: 2nd year 2nd semester
Lectures: 30 lessons/semester
Practical course: 15 lessons/semester (5×2 hours of lab. practicals, 5 hours of plenary practicals)
Practical course grade: No
Method of evaluation: midterm (general virology), exam
Teaching staff – lectures: Petra Forgách, András Marosi
Teaching staff – practical course: Petra Forgách, András Marosi
Contact: [Click to see email]
The students learn general and specific virology with a special focus on pathogens of veterinary importance. They learn the immune response of the host, its protection against infectious diseases and the most important diagnostic methods. At the practicals, the main laboratory techniques and identification procedures are demonstrated together with the characteristics and identification of the most important viruses.
Teaching is organised in the form of lectures, plenary practicals, and small-group (16 students/group) laboratory practicals.
The computer-aided lectures and plenary practicals are given as scheduled in the timetable in the lecture theatres of the campus and streamed online in Teams. After the lectures, the PPT notes and recorded videos are shared in Moodle.
In the 2020/2021 academic year, regardless of the pandemic situation, the laboratory practicals are held online by narrated PPTs and pre-recorded videos shared in Moodle. The demonstration material is prepared in the Virology laboratory of the Department. The theoretical background of the laboratory practicals is explained at the plenary lectures and in written practical notes uploaded in Moodle.
- The ICTV Report https://talk.ictvonline.org/ictv-reports/ictv_online_report/
- MACLACHLAN, N. J. – DUBOVI, E. J. (ed.): Fenner’s Veterinary Virology. 5th ed. Elsevier, 2016.
- MACLACHLAN, N. J. – DUBOVI, E. J. (ed.): Fenner’s Veterinary Virology. 4th ed. Elsevier, 2010.
- QUINN, P. J. – MARKEY, B. K. – LEONARD, F. C. – HARTIGAN, P. – FANNING, S. – FITZPATRICK, E. S.: Veterinary Microbiology and Microbial Disease. 2nd ed. Oxford: Blackwell, 2011.
Topics highlighted in bold are discussed at plenary practical dates!
|On the week of:||Topic|
|8 February 2021||Place and significance of viruses in the biosphere, evolution of viruses|
|Morphology of viruses|
|15 February 2021||General characteristics of viral nucleic acid|
|Viral proteins, their role and methods of their investigation. Viral lipids and carbohydrates|
|22 February 2021||General features of virus multiplication|
|Mutation of viruses and its role in virus evolution|
|At the plenary on 25 or 26 February||Transcription, translation and nucleic acid replication of DNA viruses|
|Transcription, translation and nucleic acid replication of RNA viruses|
|1 March 2021||Viral interactions (recombination, complementation, phenotypic mixing, interference, virus exaltation)|
|Virus-host cell interactions, viral oncogenicity, cytopathic effects and plaque formation|
|8 March 2021||General features of viral infection (infection routes, factors influencing the virus – host interactions)|
|Types of viral infections (acute, latent, tolerated, persisting and slow infections)|
|At the plenary on 11 or 12 March||Antiviral medicines, interferons, interferon inducers and immune sera in therapy|
|Immunization against viral diseases, types of viral vaccines|
|15 March 2021||Papillomaviridae, Polyomaviridae, Adenoviridae|
|22 March 2021||Poxviridae|
|5 April 2021||Parvoviridae, Circoviridae, Anelloviridae|
|12 April 2021||Picornavirales, Caliciviridae|
|19 April 2021||Togaviridae, Flaviviridae|
|Nidovirales I (Arteriviridae)|
|26 April 2021||Nidovirales II (Coronaviridae)|
|3 May 2021||Orthomyxoviridae|
|10 May 2021||Rhabdoviridae, Filoviridae, Bornaviridae|
|17 May 2021||Bacteriophages|
Practical lessons theme
Topics of plenary practicals:
|Date||Virology plenary practicals|
|11 Feb. / 12 Feb. 2021||Laboratory diagnosis of viral infections (collection and transport of samples).|
|Direct demonstration of virus infections I: virus isolation.|
|18 Feb. / 19 Feb. 2021||Direct demonstration of virus infections II: virus identification, detection of viral antigens.|
|Titration of viruses (infective titer, hemagglutinating titer).|
|4 March / 5 March 2021||Direct demonstration of virus infections III: detection of viral nucleic acid.|
|Detection and titration of antiviral antibodies (serumneutralization, hemagglutination inhibition).|
In the 2020/2021 academic year, regardless of the pandemic situation, the laboratory practicals are held online by narrated PPTs and pre-recorded videos shared in Moodle. The deadline to complete all 5 practicals is 28 March 2021.
Topics of laboratory practicals:
|1||Virus isolation from diagnostic samples (inoculation of embryonated eggs, and cell cultures)|
|2||Virus-induced cytopathic effects and plaque-formation on cell cultures, haemagglutination test|
|3||Direct diagnosis of viral infections using molecular methods I. (virus nucleic acid purification, polymerase chain reaction)|
|4||Direct diagnosis of viral infections using molecular methods II. (agarose gel electrophoresis), indirect diagnosis of viral infections I. (virus neutralisation test)|
|5||Indirect diagnosis of viral infections II. (evaluation of virus neutralisation, haemagglutination inhibition)|
Teaching, requirements of the subject
- Lectures serve as the basis of the subject, active participation at the lectures is the precondition of a successful exam. The online, real-time attendance at the lectures, due to the opportunity of interactive teaching (e.g., instant questions from the students), is highly recommended.
- The draft lecture notes in itself are not enough for the preparation for the successful exam! Previous year’s lecture notes in pdf format can be downloaded in advance from Moodle – printing and supplementing these drafts with personal notes by the information discussed at the lectures is strongly advised.
- Lecture dates and venues:
- 2nd year International class groups 1-4: Wednesday 8:15-10:00 Kotlán (Parasitology) lecture hall
- 2nd year International class groups 5-10: Wednesday 10:15-12:00 Kotlán (Parasitology) lecture hall
- Those who are exempted from “General virology” (including the practicals as well) need to attend the “Specific virology” lectures starting on the week of 15 March 2021 (Topic: Papillomaviridae, Polyomaviridae, Adenoviridae).
- The practicals include 5 hours of plenary practicals and 10 hours of laboratory practicals.
- Laboratory practicals are – regardless of the pandemic situation – conducted online, scheduled in the timetable only on administrative grounds. Therefore, there is no reason for group change. For the easy handling of the Moodle system, in the registration to plenary and practical groups please, follow the Neptun recommendation, i.e. sign up to practical groups (up to 16 students/group) appertain to the chosen plenary group.
- At the plenary practicals, the theoretical background of the laboratory practical is reviewed. The online, real-time attendance at the lectures, due to the opportunity of interactive teaching (e.g., instant questions from the students), is highly recommended. The laboratory practical teaching materials will be available after the plenary notes are (at least) opened in Moodle.
- The accomplishment of each laboratory practicals is checked by a test with questions based on the notes of the relevant plenary practical, the laboratory practical handout, PPTs and pre-recorded videos.
- To answer the 10 multiple-choice questions 5 minutes are available, each practical test can be started 3 times. To complete a laboratory practical, 5 out of 10 questions should be answered correctly (50%) in the test (i.e., among the 3 attempts at least once 5 correct answers are needed at each practical).
- The deadline to complete all 5 practicals is 28 March 2021.
- The accomplishment of all 5 practicals is the condition on the acceptance of the semester certified by signature at the black book and therefore the condition of registration to the exam.
- Those who are exempted from “General virology” including the practicals as well, do not need to sign up to plenary and practical groups. Those, who are exempted from “General virology” but required to complete the practicals, should sign up to plenary and practical groups.
- At the end of the “General Virology” part of the course, an online midterm is written. Types of questions include true/false and multiple-choice test. The midterm aims to establish the knowledge necessary for the understanding of the “Specific Virology” part of the course.
- Midterm date: 7 April 2021 (Wednesday) 18:00.
- The basis of the midterm are the lecture notes, plenary practical notes, laboratory practical handouts, PPTs and pre-recorded videos. Passing score is 50%.
- There is only 1 midterm opportunity (one date), no second chance (re-take date).
- If the result of the midterm is at least “passing” (grade 2), it is included in the final mark of the exam at the end of the semester, i.e., at the final exam, only the specific virology test should be completed. In case of failing at the midterm, both general and specific virology topics are included in the exam questions.
- Those who are exempted from “General virology” are not required to write the midterm.
- Students can sit an examination both after the spring and autumn semesters. Condition of the registration to the exam is the signature at the black book.
- Exam days are determined by the Department before the start of the exam period. Registration to exam dates and postponing an exam is to be done at Neptun system.
- The online exam consists of true/false and multiple-choice test questions, the passing score is 50%.
- The results of the written exam will be published in Neptun within 5 days after the exam.
- In case of failing at the exam, re-take is possible by the exam rules of the University.